casein solution (Thermo Fisher)
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Thermo Fisher
casein solution
Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casein+blocking+buffer/Casein+Blocking+Buffer%2C+3%25+in+PBS/10__1080_slash_0886022x__2026__2676320-67-83-87
Average 95 stars, based on 1 article reviews
Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casein+blocking+buffer/Casein+Blocking+Buffer%2C+3%25+in+PBS/10__1080_slash_0886022x__2026__2676320-67-83-87
Average 95 stars, based on 1 article reviews
casein solution - by Bioz Stars,
2026-09
95/100 stars
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Blocking Assay:Article Title: ROS-dependent localization of glycolytic enzymes to mitochondria Article Snippet: Protein samples (20 μg protein) were fractionated by SDS-PAGE using casted or pre-cast gels (Bio-Rad, 8–16 % Mini-PROTEAN TGX Precast Protein Gels) and transferred onto polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Immobilon-P PVDF Membrane, 0.45 μm pore size) using Tris-glycine transfer buffer (Cell Signaling Technology) for 1 h at 100 V at 4 °C. .. Membranes were blocked with Article Title: Endemic coronavirus infection is associated with SARS-CoV-2 Fc receptor-binding antibodies Article Snippet: SARS-CoV-2 RBD protein (SinoBiological, 40592-V08H), SARS-CoV-2 S2 protein (SinoBiological, 40590-V08H1), SARS-CoV-2 nucleocapsid protein (SinoBiological, 40588-V08B), HCoV-OC43 spike protein (SinoBiological, 40607-V08B), HCoV-OC43 nucleocapsid protein (SinoBiological, 40643-V07E), HCoV-229E spike protein (SinoBiological, 40605-V08B), HCoV-229E nucleocapsid protein (SinoBiological, 40640-V07E), HCoV-HKU1 nucleocapsid protein (SinoBiological, 40642-V07E), or HCoV-NL63 nucleocapsid protein (SinoBiological, 40641-V07E) was used in these ELISAs. .. After overnight incubation with an antigen, wells were blocked with Article Title: Knockout of Perilipin-2 in Microglia Alters Lipid Droplet Accumulation and Response to Alzheimer’s Disease Stimuli Article Snippet: Equal protein (15 μg) was denatured in 4× Laemmli buffer with 5% β-mercaptoethanol (95 °C, 5 min), separated on 4–20% Criterion TGX precast gels (Bio-Rad Laboratories, Hercules, CA, USA; 5671094) at 180 V for 50–65 min, and transferred to nitrocellulose using Trans-Blot Turbo midi transfer packs (Bio-Rad Laboratories, Hercules, CA, USA; 17041590) with the manufacturer’s rapid program (~7 min). .. Membranes were blocked in Article Title: Fusion of Complement Fragment C3d Enhances Germinal Center Responses to HIV-1 Envelope Glycoproteins Article Snippet: Galanthus nivalis lectin (Vector Laboratories) was coated onto half-area 96-well plates at a concentration of 20 μg/mL in 0.1 M NaHCO 3 (pH 8.6) and incubated overnight at room temperature (RT). .. Plates were washed with TBS and blocked for 30 min using Article Title: Compounds and methods targeting epiregulin Article Snippet: Mono Fc EREG fusions (see above) were diluted to 1 μg/mL in PBS, and 100 μL was added to each well to coat an ELISA plate (Greiner Cat #655061) overnight at 5° C. Variants were grouped by column on the plate with wild type coated in 3 replicate columns and the point mutants coated in single replicate columns. .. Following the coat, the plate was washed 3×200 μL per well with PBST, then blocked at RT for 1 hour on a plate shaker using Article Title: A conserved region T-cell vaccine for Sarbecoviruses Article Snippet: Briefly, 100 ng/well of whole spike protein (BEI-Resources NR-55170) in PBS was coated in an ELISA plate (Immulon 2HB) at 4 0 C overnight. .. The plate was blocked with Saline:Article Title: ROS-dependent localization of glycolytic enzymes to mitochondria Article Snippet: Protein samples (20 μg protein) were fractionated by SDS-PAGE using casted or pre-cast gels (Bio-Rad, 8–16 % Mini-PROTEAN TGX Precast Protein Gels) and transferred onto polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Immobilon-P PVDF Membrane, 0.45 μm pore size) using Tris-glycine transfer buffer (Cell Signaling Technology) for 1 h at 100 V at 4 °C. .. Membranes were blocked with Incubation:Article Title: ROS-dependent localization of glycolytic enzymes to mitochondria Article Snippet: Protein samples (20 μg protein) were fractionated by SDS-PAGE using casted or pre-cast gels (Bio-Rad, 8–16 % Mini-PROTEAN TGX Precast Protein Gels) and transferred onto polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Immobilon-P PVDF Membrane, 0.45 μm pore size) using Tris-glycine transfer buffer (Cell Signaling Technology) for 1 h at 100 V at 4 °C. .. Membranes were blocked with Article Title: Endemic coronavirus infection is associated with SARS-CoV-2 Fc receptor-binding antibodies Article Snippet: SARS-CoV-2 RBD protein (SinoBiological, 40592-V08H), SARS-CoV-2 S2 protein (SinoBiological, 40590-V08H1), SARS-CoV-2 nucleocapsid protein (SinoBiological, 40588-V08B), HCoV-OC43 spike protein (SinoBiological, 40607-V08B), HCoV-OC43 nucleocapsid protein (SinoBiological, 40643-V07E), HCoV-229E spike protein (SinoBiological, 40605-V08B), HCoV-229E nucleocapsid protein (SinoBiological, 40640-V07E), HCoV-HKU1 nucleocapsid protein (SinoBiological, 40642-V07E), or HCoV-NL63 nucleocapsid protein (SinoBiological, 40641-V07E) was used in these ELISAs. .. After overnight incubation with an antigen, wells were blocked with Article Title: Knockout of Perilipin-2 in Microglia Alters Lipid Droplet Accumulation and Response to Alzheimer’s Disease Stimuli Article Snippet: Equal protein (15 μg) was denatured in 4× Laemmli buffer with 5% β-mercaptoethanol (95 °C, 5 min), separated on 4–20% Criterion TGX precast gels (Bio-Rad Laboratories, Hercules, CA, USA; 5671094) at 180 V for 50–65 min, and transferred to nitrocellulose using Trans-Blot Turbo midi transfer packs (Bio-Rad Laboratories, Hercules, CA, USA; 17041590) with the manufacturer’s rapid program (~7 min). .. Membranes were blocked in Membrane:Article Title: In Vitro and In Vivo Comparative Analysis of Muscle Regenerative Processes Induced by Different Microcurrent Waveforms in Skeletal Muscle Atrophy Article Snippet: The quantified structures were segregated via SDS–polyacrylamide gel electrophoresis, with NuPAGE 4–12% bis-Tris gels (Invitrogen, Carlsbad, CA, USA), and then transferred to a 0.2 μm PVDF membrane (Cytiva, Marborough, MA, USA). .. The membrane was fixed with a Article Title: In Vitro and In Vivo Comparative Analysis of Muscle Regenerative Processes Induced by Different Microcurrent Waveforms in Skeletal Muscle Atrophy Article Snippet: The quantified structures were segregated via SDS–polyacrylamide gel electrophoresis, with NuPAGE 4–12% bis-Tris gels (Invitrogen, Carlsbad, CA, USA), and then transferred to a 0.2 μm PVDF membrane (Cytiva, Marborough, MA, USA). .. The membrane was fixed with a |